Dept. of Biotechnology

Biochemistry and Instrumentation Laboratory   

This lab provides an in-depth first hand knowledge of various instrumental techniques, with special emphasis on real time spectrometers. Experiments for both qualitative and quantitative analysis of bimolecular proteins like DNA, RNA etc are carried out with the aid of a wide variety of equipment like colorimeters, spectrophotometers, fluorimeters, conductivity meters etc.,. Separation techniques like paper, column and thin layer chromatography are also practiced. Extraction of few medicinally important compounds like caffeine helps the students to have an idea of the usage and advantage of techniques like refluxing and vacuum distillation.

Microbial Technology Laboratory   

This lab will enable students to understand and describe staining characteristics, media selection, incubation conditions, colonial morphology, biochemical and serological reactions, and antibiotic sensitivity patterns of routine Bacteria, Viruses, and Parasites etc. students gain acquaintance with the microbial technology skills by working with state of art equipment like Laminar Flow Chamber, Orbital Shaking Incubator, Vertical Autoclave etc,.

Cell biology Laboratory   

Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. Cell culture conditions can vary for each cell type, but artificial environments consist of a suitable vessel with substrate or medium that supplies the essential nutrients (amino acids, carbohydrates, vitamins, minerals), growth factors, hormones, and gases (CO2, O2), and regulates the physio-chemical environment (pH buffer, osmotic pressure, temperature). So in this lab, students are trained and acquainted with preparation of growth media, collection of cells from embryos and adult organisms. Further students gets hands on experience on techniques viz., trypsinization, passaging of cells for further generations and measuring cell viability & cell death. At the end of this laboratory students will be capable of growing animal cells under aseptic conditions and test molecular cytotoxicity.

Molecular Biology and Genetic Engineering Laboratory   

The objective of this lab is to provide a working knowledge of fundamental and advanced techniques like PCR, Blotting Techniques and Electrophoresis etc., that are used for the separation of amplification and cloning of isolated DNA into various types of vectors. The screening of such recombinants is carried out. Also the demonstration of mutagenesis helps the students to understand the mutation phenomenon.

Tissue Culture and Immunology Laboratory   

In this laboratory students will be taught to carry out in vitro creation of new plantlets and thereby learn the art and science of plant multiplication. With the aid of available modern trend and instruments, students will practice the various steps involved in the application of this technology like - stock plant care, explant selection and sterilization, media manipulation to obtain proliferation, rooting, acclimatization and growing in sterile cultures to maintain parent lines. Students are exposed to clinical medicine and will be assigned laboratory exercises for acquainting them with the latest advances in immunological techniques today.

Bioprocess Engineering and Downstream Processing Laboratory   

In this lab, the high yield producing biocatalysts & enzymes are extracted and an assay of them is carried out. Students learn to understand & determine the Optimal conditions for the usage of enzymes towards the profitable running of industries. Expertise on various immobilizing techniques is imparted for the easy separation, recycle and reuse of enzymes. Students will also practise various industrial level downstream processing techniques like cooling centrifugation, plate & frame filtration, shell & leaf filtration in addition to learning general laboratory scale techniques like chromatography, sedimentation, precipitation etc.

Bioinformatics   

The laboratory has a well organised and independent computer set up with 24 hours high speed internet connection for carrying out practicals in various areas of Bioinformatics, like computer programming , access to Bioinformatics tools, structure visualization, structure modeling etc. Students also use the facility to carry out academic projects related to the field.

Supporting Laboratories   

Chemical Reaction Engineering Lab   

Students understand the performance characteristics and the advantages and disadvantages of major reactor types. Principles of chemical reactor analysis and design. Experimental determination of rate equations, design of batch and continuous reactors.

Mass Transfer Operations Lab   

This laboratory is associated with the course mass transfer operations. It provides an opportunity for the students to conduct experiments relating to various ways of separation techniques. and also determine the overall mass transfer coefficient by conducting the extraction, absorption and adsorption techniques.

Instrumentation and Process Control Lab   

Understand the dynamic characteristic of the first order and second order systems by taking some examples of the systems, first order systems connected in series-interacting and non-interacting systems.

Fluid Mechanics and Heat Transfer Lab   

This lab consists of several experimental setups for verifying the basic laws of fluid mechanics and some flow measuring devices, also helps to learn the working principle of the different types of pumps. Observing the nature of the heat flow, and understanding the working principle of conduction, convection and radiation.

List of Facilities in CoE
Instrument: High Performance Liquid Chromatography

High performance Liquid Chromatography is a powerful tool popularly used in analysis of chemical compounds. It allows the user to spend minimal sample size and reap maximum results. The chemical compounds are analysed by high-efficient and sensitive photo diode array detector (DAD).

Instrument: UV-Vis Spectrophotometer

Cary 60 is a cuvette based versatile spectrophotometer capable of reading samples from 190 nm to 1100 nm with 1 nm increment. The system is also fitted with Peltier device for temperature regulation ranging from 0 OC to 100 OC to study temperature dependent chemical kinetics.

Instrument: Fluorescence spectrophotometer

Cary eclipse is a cuvette based highly sensitive and efficient fluorescent spectrophotometer. It can handle fluorescence, phosphorescence, chemi/bio-luminescence, and time resolved phosphorescence measurements very easily. The software is very user friendly and application specific.

Instrument: Inverted Fluorescence microscope

Inverted fluorescence microscope is a user-friendly and ergonomic tool for visualising animal cells under fluorescence and phase contrast fields. The microscope is fitted with 4 different filters viz., red, blue, green and violet that covers a wide range of fluorophores and gives the user a greater choice for dye selection. It has got high-quality objecitves-4X, 10X, 20X and 40X. The stage of the microscope is interchangeable to accommodate different kinds of culture wares.

Instrument: Upright Fluorescence Microscope

It is an ergonomic bright field and fluorescence microscope used for teaching, training and routine applications. It is fitted with 4X, 10X, 40X and 100X (oil immersion) objectives. Two fluorescence filters-blue and green provide fluorescence fields to work with different experiments. The microscope is also fitted with Olympus digital camera for efficient digital imaging and documentation.

Instrument: Thermal cycler

The Eppendorf Mastercycler nexus gradient is a gradient thermal cycler used for amplication of desired gene from complex genetic material. This machine has a gradient range of 4-990C and 96x 0.2 ml PCR tubes or 77x 0.5 ml PCR tubes or 1x 96 well PCR plate capacity.

Instrument: Thermal cycler

Surecycler8800 is a gradient thermal cycler that supports 12 temperature gradient over 12 columns. It offers both fast cycling and supports large volumes upto 100 uL. This can run even the most complex thermal cycling techniques including time and temperature increments and touchdown PCR.

Instrument: Microtiter Plate Reader

ELx800 is a filter based absorbance reader for 96 well plates. It measures optical density, absorbance and transmittance at 405nm, 490nm and 630nm. It is suitable for applications in experiments pertaining to clinical, biotechnology research and pharmaceutical laboratory.

Instrument: Microtiter Plate Reader

Multiskan FC is a monochromator based reader capable of reading 96 well and 384 well plates at wavelengths ranging from 340 nm to 850 nm. It is also equipped with shaking and incubation up to 50 OC for kinetics studies. Various assays such as Immunoassays (ELISA), protein assays, endotoxins, cytotoxicity and proliferation assays, enzyme assays and growth curves can be performed with ease using the SkanItTM Software. Instrument: Sonicator

Sonicator is a probe equipment used for efficient disruption of materials. It is suitable for breaking Bacterial cell, bacterial spores, Animal & plant Cells. Also used for degassing, emulsifying, Cleaning, Mixing etc.

Instrument: UV-VIS Spectrophotometer

UV-1800 is a compact cuvette based UV-VIS spectrophotometer that can be used either as a stand-alone instrument or as a PC-controlled instrument. It offers high resolution of 1nm wavelength reading ranging from 180 nm to 1100 nm. Users cananalyse data on a PC using UVProbe software. In addition, data for spectra and time-course curves can be displayed and saved with commercial spreadsheet software.

Instrument: Fermenter

Fermenter is a stainless steel reactor ergonomically designed for culturing large amounts of bacteria, yeast and animal cells under controlled conditions for production of biomolecules such as antibiotics, antibodies, proteins and enzymes.

Instrument: CO2 incubator

CO2 incubator is a high-capacity, 170 liter incubator that provides high quality incubation within a minimal footprint to grow animal cells with temperature range from 40C above ambient to 500C.M

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